Clinically relevant, orthotopically-developed GBM models enable accurate assessment of a therapeutic’s interaction with the blood-brain barrier (BBB), including permeability and transport. Orthotopic patient-derived xenograft (O-PDX) models have been shown to retain the same genomic, histological, epigenetic, and treatment response characteristics as parental tumors and identify the gene signatures and pathways signatures associated with the clinical aggressiveness of GBMs.1,2
Download our GBM data summary to learn more about our offerings, including in vitro and in vivo irradiation, as well as our well-characterized orthotopic intracranial GBM 3D spheroid models, with patient history, treatment history; survival rates, growth kinetics, growth sensitivity and stem cell marker data.
Specialized intracranial microsurgery expertise and instrumentation results in speed, precision and a > 90% GBM tumor take rate.3 Certis skilled microsurgeons use extreme care to promote homeostasis within the brain, using stereotactic implantation to optimize placement of cells in brain tissue, and convection-enhanced delivery (CED) to optimize placement of tested compounds in brain tissue. A minimally invasive, single surgery allows for testing of cellular therapeutics, CAR-T's, small molecule inhibitors and chemotherapies and is associated with low (<5%) perioperative mortality.3
Imaging and analytical techniques precisely measure therapeutic response, providing visual proof when a drug formulation successfully crosses the BBB and regresses GBM tumors.
Our high-resolution technologies are non-invasive, allowing animals to be evaluated with little disruption. A highly sensitive optical bioluminescence and fluorescence imaging system (using one cell line) is useful in analyzing/measuring GBM drug effects (or how much actually crosses the BBB) while murine-scale MRIs deliver objective, precise tumor volume measurements (TVMs).
Validation of Orthotopic vs. Subcutaneous In Vivo Tumor Growth Model in Mice. A comparison of variable tumor growth in mice without any treatment using patient-derived GBM spheroids from primary or recurrent tumors excised from the same patient.4
Certis Oncology Solutions is a life science technology company committed to realizing the promise of precision oncology. Our product is Oncology Intelligence™ — highly predictive therapeutic response data derived from advanced biological models of cancer. Our proprietary platform informs individual treatment decisions and accelerates the development of new cancer therapies.
We partner with therapeutics developers to help close the problematic translation gap between preclinical studies and clinical trials. Certis brings greater certainty to go/no-go development decisions through more clinically relevant, well-characterized and annotated models combined with advanced imaging technology. Every preclinical study Certis designs with you is rooted in collaboration, scientific resolve, a bias for precision and a close connection to the patient.
REFERENCES: 1 Patrizii M, Bartucci M, Pine SR, Sabaawy HE. Utility of Glioblastoma Patient-Derived Orthotopic Xenografts in Drug Discovery and Personalized Therapy. Front Oncol. 2018;8:23. 2 Joo KM, Kim J, Jin J, et al. Patient-Specific Orthotopic Glioblastoma Xenograft Models Recapitulate the Histopathology and Biology of Human Glioblastomas In Situ. Cell Rep. 2013;3(1):260-273.] 3 Baumann BC, Dorsey JF, Benci JL et al. Stereotactic Intracranial Implantation and In Vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme. J Vis Exp. 2012;(67):4089. 4 Rajan RG, Fernandez-Vega V, Sperry J, Nakashima J, Do LH, Andrews W, Boca S, Islam R, Chowdhary SA, Seldin J, et al. In Vitro and In Vivo Drug-Response Profiling Using Patient-Derived High-Grade Glioma. Cancers. 2023; 15(13):3289.